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m will not be minimal through the Affiliation fee consistent to the development of the NagA-substrate complicated. These results (along with the improved fee of response of compound six relative to compound 1) are only in line with the summary that NagA is restricted by possibly the formation or breakdown of your tetrahedral intermediate.
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cat vs. pH profile, one ionization is noticed for either Zn- or Cd-NagA. This ionization must symbolize the protonation with the metallic-bound hydroxide that is utilized for substrate hydrolysis or, alternatively, the general base that may be used to deprotonate the metallic-certain drinking water molecule just before substrate hydrolysis. The p
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The metallic material and also the corresponding kinetic parameters for your metal-substituted variants of NagA are detailed in Table 1.
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